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[KD Validated] LAMP1 Rabbit pAb (A16894)

Publications (14) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] LAMP1 Rabbit pAb (A16894)

Western blot analysis of lysates from wild type (WT) and LAMP1 knockdown (KD) HeLa cells using [KD Validated] LAMP1 Rabbit pAb (A16894) at 1:2000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KD Validated] LAMP1 Rabbit pAb (A16894)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using LAMP1 Rabbit pAb (A16894) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] LAMP1 Rabbit pAb (A16894)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using LAMP1 Rabbit pAb (A16894) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] LAMP1 Rabbit pAb (A16894)

Immunohistochemistry analysis of paraffin-embedded Human lung tissue using LAMP1 Rabbit pAb (A16894) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] LAMP1 Rabbit pAb (A16894)

Immunofluorescence analysis of BALA-3T3 cells using LAMP1 Rabbit pAb (A16894) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name[KD Validated] LAMP1 Rabbit pAb
Catalog No.A16894
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 150-250 of human LAMP1 (NP_005552.3).
SequenceDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIV
Gene ID
Swiss Prot
SynonymsLAMPA; CD107a; LGP120; LAMP1
Calculated MW45kDa
Observed MW42kDa/90-120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa
Cellular locationCell membrane, Endosome membrane, Late endosome, Lysosome membrane, Single-pass type I membrane protein.
Customer validation

WB(Homo sapiens, Mus musculus, Mus musculus)

IF(Homo sapiens, Rattus norvegicus)

IHC(Mus musculus)

IF(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KD Validated] LAMP1 Rabbit pAb (A16894)}

Western blot - [KD Validated] LAMP1 Rabbit pAb (A16894)

Western blot analysis of lysates from wild type (WT) and LAMP1 knockdown (KD) HeLa cells using [KD Validated] LAMP1 Rabbit pAb (A16894) at 1:2000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KD Validated] LAMP1 Rabbit pAb (A16894)}

Immunohistochemistry - [KD Validated] LAMP1 Rabbit pAb (A16894)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using LAMP1 Rabbit pAb (A16894) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] LAMP1 Rabbit pAb (A16894)}

Immunohistochemistry - [KD Validated] LAMP1 Rabbit pAb (A16894)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using LAMP1 Rabbit pAb (A16894) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] LAMP1 Rabbit pAb (A16894)}

Immunohistochemistry - [KD Validated] LAMP1 Rabbit pAb (A16894)

Immunohistochemistry analysis of paraffin-embedded Human lung tissue using LAMP1 Rabbit pAb (A16894) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KD Validated] LAMP1 Rabbit pAb (A16894)}

Immunofluorescence - [KD Validated] LAMP1 Rabbit pAb (A16894)

Immunofluorescence analysis of BALA-3T3 cells using LAMP1 Rabbit pAb (A16894) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (7)

재조합 단백질 (1)

Secondary Antibodies (26)