Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from HepG2 cells, using Cytokeratin 7 (KRT7) Rabbit pAb (A12004) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of lysates from Rat lung, using Cytokeratin 7 (KRT7) Rabbit pAb (A12004) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Cytokeratin 7 (KRT7) Rabbit pAb (A12004) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of human lung cancer using Cytokeratin 7 (KRT7) Rabbit pAb (A12004) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse bronchus using Cytokeratin 7 (KRT7) Rabbit pAb (A12004) at dilution of 1:100. Blue: DAPI for nuclear staining.
| Product name | Cytokeratin 7 (KRT7) Rabbit pAb |
|---|---|
| Catalog No. | A12004 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-94 of human Cytokeratin 7 (KRT7) (NP_005547.3). |
|---|---|
| Sequence | MSIHFSSPVFTSRSAAFSGRGAQVRLSSARPGGLGSSSLYGLGASRPRVAVRSAYGGPVGAGIREVTINQSLLAPLRLDADPSLQRVRQEESEQ |
| Gene ID | |
| Swiss Prot | |
| Synonyms | K7; CK7; SCL; K2C7; Cytokeratin 7 (KRT7) |
| Calculated MW | 51kDa |
| Observed MW | 51kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | HepG2, Rat lung |
| Cellular location | Cytoplasm |
| Customer validation | WB(Homo sapiens) IF(Homo sapiens, Mus musculus) Other(Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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