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Cytokeratin 7 (KRT7) Rabbit mAb (A4765)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)

Western blot analysis of various lysates using Cytokeratin 7 (KRT7) (KRT7) Rabbit mAb (A4765) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)

Western blot analysis of various lysates using Cytokeratin 7 (KRT7) (KRT7) Rabbit mAb (A4765) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)

Immunohistochemistry analysis of paraffin-embedded Human breast using Cytokeratin 7 (KRT7) Rabbit mAb (A4765) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)

Immunohistochemistry analysis of paraffin-embedded Human lung using Cytokeratin 7 (KRT7) Rabbit mAb (A4765) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)

Immunohistochemistry analysis of paraffin-embedded Human pancreas using Cytokeratin 7 (KRT7) Rabbit mAb (A4765) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Cytokeratin 7 (KRT7) Rabbit mAb (A4765) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)

Immunofluorescence analysis of paraffin-embedded human lung using Cytokeratin 7 (KRT7) Rabbit mAb (A4765) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCytokeratin 7 (KRT7) Rabbit mAb
Catalog No.A4765
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1267
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cytokeratin 7 (KRT7) (P08729).
SequenceMSIHFSSPVFTSRSAAFSGRGAQVRLSSARPGGLGSSSLYGLGASRPRVAVRSAYGGPVGAGIREVTINQSLLAPLRLDADPSLQRVRQEESEQIKTLNN
Gene ID
Swiss Prot
SynonymsK7; CK7; SCL; K2C7; Cytokeratin 7 (KRT7)
Calculated MW51kDa
Observed MW52kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • IHC-P 1:200 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, A-549, Mouse lung, Mouse kidney, Rat kidney
Cellular locationCytoplasm.
Customer validation

IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)}

Western blot - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)

Western blot analysis of various lysates using Cytokeratin 7 (KRT7) (KRT7) Rabbit mAb (A4765) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)}

Western blot - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)

Western blot analysis of various lysates using Cytokeratin 7 (KRT7) (KRT7) Rabbit mAb (A4765) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunohistochemistry - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)}

Immunohistochemistry - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)

Immunohistochemistry analysis of paraffin-embedded Human breast using Cytokeratin 7 (KRT7) Rabbit mAb (A4765) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)}

Immunohistochemistry - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)

Immunohistochemistry analysis of paraffin-embedded Human lung using Cytokeratin 7 (KRT7) Rabbit mAb (A4765) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)}

Immunohistochemistry - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)

Immunohistochemistry analysis of paraffin-embedded Human pancreas using Cytokeratin 7 (KRT7) Rabbit mAb (A4765) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)}

Immunohistochemistry - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Cytokeratin 7 (KRT7) Rabbit mAb (A4765) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)}

Immunofluorescence - Cytokeratin 7 (KRT7) Rabbit mAb (A4765)

Immunofluorescence analysis of paraffin-embedded human lung using Cytokeratin 7 (KRT7) Rabbit mAb (A4765) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)