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Ubiquityl-Histone H2B-K120 Rabbit pAb (A17949)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

Overview

Product name	Ubiquityl-Histone H2B-K120 Rabbit pAb
Catalog No.	A17949
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.H2B clustered histone (H2BC) consists of several H2B family members, which function as replication-dependent histone proteins.

Immunogen information

Immunogen	A synthetic ubiquitinated peptide around K120 of human HIST1H2BC (NP_066407.1).
Sequence	VTKYT
Gene ID
Swiss Prot
Synonyms	HIST1H2BC; Ubiquityl-Histone H2B-K120
Calculated MW	14kDa
Observed MW	Refer to figures

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples
Cellular location	cytosol, extracellular exosome, extracellular space, nucleoplasm, nucleus.