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Formyl-Histone H2B-K120 Rabbit mAb (A0065)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Formyl-Histone H2B-K120 Rabbit mAb (A0065)

Western blot analysis of various lysates using (A0065) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)

Immunohistochemistry analysis of Formyl-Histone H2B-K120 in paraffin-embedded mouse brain tissue using Formyl-Histone H2B-K120 Rabbit mAb (A0065) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)

Immunohistochemistry analysis of Formyl-Histone H2B-K120 in paraffin-embedded rat liver tissue using Formyl-Histone H2B-K120 Rabbit mAb (A0065) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)

Immunohistochemistry analysis of Formyl-Histone H2B-K120 in paraffin-embedded human thyroid tissue using Formyl-Histone H2B-K120 Rabbit mAb (A0065) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)

Immunohistochemistry analysis of Formyl-Histone H2B-K120 in paraffin-embedded human liver tissue using Formyl-Histone H2B-K120 Rabbit mAb (A0065) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)

Immunohistochemistry analysis of Formyl-Histone H2B-K120 in paraffin-embedded mouse testis tissue using Formyl-Histone H2B-K120 Rabbit mAb (A0065) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)

Immunohistochemistry analysis of Formyl-Histone H2B-K120 in paraffin-embedded rat spleen tissue using Formyl-Histone H2B-K120 Rabbit mAb (A0065) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)

Immunohistochemistry analysis of Formyl-Histone H2B-K120 in paraffin-embedded rat testis tissue using Formyl-Histone H2B-K120 Rabbit mAb (A0065) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameFormyl-Histone H2B-K120 Rabbit mAb
Catalog No.A0065
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2493
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity.
ImmunogenA synthesized peptide derived from human Histone H2B (formyl K120).
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsH2B; H2BE; H2BQ; GL105; H2B.1; H2BFQ; H2BGL105; H2B-GL105; HIST2H2BE; Formyl-Histone H2B-K120
Calculated MW14kDa
Observed MW15kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, NIH/3T3, C6
Cellular locationChromosome, Nucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Formyl-Histone H2B-K120 Rabbit mAb (A0065)}

    Western blot - Formyl-Histone H2B-K120 Rabbit mAb (A0065)

    Western blot analysis of various lysates using (A0065) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)}

    Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)

    Immunohistochemistry analysis of Formyl-Histone H2B-K120 in paraffin-embedded mouse brain tissue using Formyl-Histone H2B-K120 Rabbit mAb (A0065) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)}

    Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)

    Immunohistochemistry analysis of Formyl-Histone H2B-K120 in paraffin-embedded rat liver tissue using Formyl-Histone H2B-K120 Rabbit mAb (A0065) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)}

    Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)

    Immunohistochemistry analysis of Formyl-Histone H2B-K120 in paraffin-embedded human thyroid tissue using Formyl-Histone H2B-K120 Rabbit mAb (A0065) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)}

    Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)

    Immunohistochemistry analysis of Formyl-Histone H2B-K120 in paraffin-embedded human liver tissue using Formyl-Histone H2B-K120 Rabbit mAb (A0065) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)}

    Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)

    Immunohistochemistry analysis of Formyl-Histone H2B-K120 in paraffin-embedded mouse testis tissue using Formyl-Histone H2B-K120 Rabbit mAb (A0065) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)}

    Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)

    Immunohistochemistry analysis of Formyl-Histone H2B-K120 in paraffin-embedded rat spleen tissue using Formyl-Histone H2B-K120 Rabbit mAb (A0065) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)}

    Immunohistochemistry - Formyl-Histone H2B-K120 Rabbit mAb (A0065)

    Immunohistochemistry analysis of Formyl-Histone H2B-K120 in paraffin-embedded rat testis tissue using Formyl-Histone H2B-K120 Rabbit mAb (A0065) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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