제품 > 항체 > 단일클론항체(mAb)

MHC Class II (I-A/I-E) Rat mAb (A26039)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - MHC Class II (I-A/I-E) Rat mAb (A26039)

Flow cytometry: 1X10^6 Neuro-2a cells (negative control, left) and C57BL/6 mouse splenocytes (right) were surface-stained with MHC Class II (I-A/I-E) Rat mAb (A26039, 2 μg/mL, orange line) or Rat IgG isotype control (2 μg/Test, blue line), followed by PE goat anti-Rat pAb staining. Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - MHC Class II (I-A/I-E) Rat mAb (A26039)

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with Rat IgG isotype control (2 μg/Test, left) or MHC Class II (I-A/I-E) Rat mAb (A26039, 2 μg/mL, right) , followed by PE goat anti-Rat pAb staining.

Overview

Product nameMHC Class II (I-A/I-E) Rat mAb
Catalog No.A26039
Host speciesRat
Purification methodAffinity purification
IsotypeIgG
CloneNo.ATC50002
Predicted to enable several functions, including MHC class II receptor activity; peptide antigen binding activity; and signaling receptor binding activity. Involved in response to interferon-gamma. Acts upstream of or within antigen processing and presentation of exogenous peptide antigen via MHC class II. Predicted to be located in external side of plasma membrane and immunological synapse. Predicted to be part of MHC class II protein complex. Predicted to be active in late endosome membrane and lysosomal membrane. Is expressed in lung. Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); bacterial infectious disease (multiple); eye disease (multiple); and sarcoidosis (multiple). Orthologous to human HLA-DRB1 (major histocompatibility complex, class II, DR beta 1) and HLA-DRB5 (major histocompatibility complex, class II, DR beta 5).
ImmunogenRecombinant protein of mouse MHC Class II (I-A/I-E).
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsEb; Ia4; H2Eb; Ia-4; H-2Eb; IAb; Ia2; Ia-2; Abeta; H-2Ab; H2-Ab; Rmcs1; I-Abeta; Ia5; Ia-5; H-2Eb2; Iebeta2; A130038H09Rik; Ia1; H2Aa; Ia-1; H-2Aa; Aalpha; IAalpha; I-Aalpha
Calculated MW26kDa/30kDa/32kDa
Observed MWRefer to figures
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Single-pass type I membrane protein

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Flow CytoMetry - MHC Class II (I-A/I-E) Rat mAb (A26039)}

    Flow CytoMetry - MHC Class II (I-A/I-E) Rat mAb (A26039)

    Flow cytometry: 1X10^6 Neuro-2a cells (negative control, left) and C57BL/6 mouse splenocytes (right) were surface-stained with MHC Class II (I-A/I-E) Rat mAb (A26039, 2 μg/mL, orange line) or Rat IgG isotype control (2 μg/Test, blue line), followed by PE goat anti-Rat pAb staining. Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - MHC Class II (I-A/I-E) Rat mAb (A26039)}

    Flow CytoMetry - MHC Class II (I-A/I-E) Rat mAb (A26039)

    Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with Rat IgG isotype control (2 μg/Test, left) or MHC Class II (I-A/I-E) Rat mAb (A26039, 2 μg/mL, right) , followed by PE goat anti-Rat pAb staining.

    * For research use only. Not for therapeutic or diagnostic purposes.