제품 > 항체 > 단일클론항체(mAb)

PE Rat anti-Mouse MHC Class II (I-A/I-E) mAb (A26002)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - PE Rat anti-Mouse MHC Class II (I-A/I-E) mAb (A26002)

Flow cytometry: 1X10^6 Neuro-2a cells (negative control, left) and C57BL/6 mouse splenocytes (right) were surface-stained with PE Rat anti-Mouse MHC Class II (I-A/I-E) mAb (A26002, 5 μl/Test, orange line) or PE Rat IgG isotype control (2 μg/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - PE Rat anti-Mouse MHC Class II (I-A/I-E) mAb (A26002)

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with PE Rat IgG isotype control (2 μg/Test, left) or PE Rat anti-Mouse MHC Class II (I-A/I-E) mAb (A26002, 5 μl/Test, right).

Overview

Product namePE Rat anti-Mouse MHC Class II (I-A/I-E) mAb
Catalog No.A26002
Host speciesRat
Purification methodAffinity purification
IsotypeIgG
CloneNo.ATC50002-PE
Predicted to enable several functions, including MHC class II receptor activity; peptide antigen binding activity; and signaling receptor binding activity. Involved in response to interferon-gamma. Acts upstream of or within antigen processing and presentation of exogenous peptide antigen via MHC class II. Predicted to be located in external side of plasma membrane and immunological synapse. Predicted to be part of MHC class II protein complex. Predicted to be active in late endosome membrane and lysosomal membrane. Is expressed in lung. Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); bacterial infectious disease (multiple); eye disease (multiple); and sarcoidosis (multiple). Orthologous to human HLA-DRB1 (major histocompatibility complex, class II, DR beta 1) and HLA-DRB5 (major histocompatibility complex, class II, DR beta 5).
ImmunogenRecombinant protein of mouse MHC Class II (I-A/I-E).
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsEb; Ia4; H2Eb; Ia-4; H-2Eb; IAb; Ia2; Ia-2; Abeta; H-2Ab; H2-Ab; Rmcs1; I-Abeta; Ia5; Ia-5; H-2Eb2; Iebeta2; A130038H09Rik; Ia1; H2Aa; Ia-1; H-2Aa; Aalpha; IAalpha; I-Aalpha
Calculated MW30kDa/32kDa/26kDa
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationMembrane, Single-pass type I membrane protein.
    ABclonal:Flow CytoMetry - PE Rat anti-Mouse MHC Class II (I-A/I-E) mAb (A26002)}

    Flow CytoMetry - PE Rat anti-Mouse MHC Class II (I-A/I-E) mAb (A26002)

    Flow cytometry: 1X10^6 Neuro-2a cells (negative control, left) and C57BL/6 mouse splenocytes (right) were surface-stained with PE Rat anti-Mouse MHC Class II (I-A/I-E) mAb (A26002, 5 μl/Test, orange line) or PE Rat IgG isotype control (2 μg/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - PE Rat anti-Mouse MHC Class II (I-A/I-E) mAb (A26002)}

    Flow CytoMetry - PE Rat anti-Mouse MHC Class II (I-A/I-E) mAb (A26002)

    Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with PE Rat IgG isotype control (2 μg/Test, left) or PE Rat anti-Mouse MHC Class II (I-A/I-E) mAb (A26002, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.