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Rabbit Control IgG (AC005)

Publications (208) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Rabbit Control IgG
ABclonal:Immunoprecipitation - Rabbit Control IgG (AC005)

Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated using DDDDK-tag antibody (AE063). Secondary antibody: HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG Light Chain (AS061).A mock served as negative control using rabbit Control IgG（AC005）and over-expressed 293T cell lysate served as positive control.

ABclonal:Chromatin Immunoprecipitation - Rabbit Control IgG (AC005)

Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from HeLa cells, using 5 μg of Rabbit Control IgG (AC005) and CTCF Rabbit pAb (A1133). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.

Overview

Product nameRabbit Control IgG
Catalog No.AC005
Host speciesRabbit
Purification methodPotein A/G purification
IsotypeIgG
The protein encoded by this gene is a transcriptional regulator and tumor suppressor, serving as an activator of genes involved in both innate and acquired immune responses. The encoded protein activates the transcription of genes involved in the body's response to viruses and bacteria, playing a role in cell proliferation, apoptosis, the immune response, and DNA damage response. This protein represses the transcription of several other genes. As a tumor suppressor, it both suppresses tumor cell growth and stimulates an immune response against tumor cells. Defects in this gene have been associated with gastric cancer, myelogenous leukemia, and lung cancer.
ImmunogenNone
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IP 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
  • ChIP 5μg antibody for 10μg-15μg of Chromatin
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationImmunoprecipitation    
Positive samples
Cellular location
Customer validation

IP(Homo sapiens, Sus scrofa, Mus musculus, Rattus norvegicus, Xanthonycticebus pygmaeus, Bos taurus, Oryctolagus cuniculus, Mus musculus, Anatinae, Oryza sativa)

WB(Rattus norvegicus, Homo sapiens, Bos taurus, Mus musculus, Glaesserella parasuis, Cyprinus carpio, Sus scrofa, Ctenopharyngodon idellus, Mus musculus, Other)

IF(Homo sapiens, Mus musculus, Rattus norvegicus)

IHC(Mus musculus)

RIP(Mus musculus, Drosophila, Homo sapiens, Sus scrofa, Drosophila melanogaster, Oryctolagus cuniculus)

Co-IP(Homo sapiens, Homo sapiens , Mus musculus, Cricetulus barabensis, Rattus norvegicus, Other, Rattus norvegicus)

Co-IP(Homo sapiens, Mus musculus)

CUT&Tag(Homo sapiens, Oryza sativa, Other, Mus musculus, Sus scrofa)

ChIP(Capra hircus, Homo sapiens, Mus musculus, Rattus norvegicus, Arabidopsis thaliana, Drosophila melanogaster, Mus musculus, Zea mays , Oryza sativa, Canis familiaris)

ChIP-Seq(Mus musculus)

IHC(Rattus norvegicus)

WB(Rattus norvegicus, Homo sapiens, Mus musculus)

Co-IP(Homo sapiens, Drosophila melanogaster)

IP(Homo sapiens, Drosophila melanogaster, Canis familiaris, Mus musculus)

ChIP(Mus musculus)

Other(Other)

CUT&RUN(Mus musculus)

dPCR(Other)

ChIP-Seq(Canis familiaris)

RIP(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunoprecipitation - Rabbit Control IgG (AC005)}

    Immunoprecipitation - Rabbit Control IgG (AC005)

    Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated using DDDDK-tag antibody (AE063). Secondary antibody: HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG Light Chain (AS061).A mock served as negative control using rabbit Control IgG（AC005）and over-expressed 293T cell lysate served as positive control.
    ABclonal:Chromatin Immunoprecipitation - Rabbit Control IgG (AC005)}

    Chromatin Immunoprecipitation - Rabbit Control IgG (AC005)

    Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from HeLa cells, using 5 μg of Rabbit Control IgG (AC005) and CTCF Rabbit pAb (A1133). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AC005? Please let us know so that we can cite the reference in this datasheet.