| Product name | Rabbit anti-Human IL-4 mAb |
|---|---|
| Catalog No. | RM17735 |
| Catagory | Elisa Antibody Kit |
| Application | ELISA, Multiplex |
| Description | The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 25-153 of human IL-4 (NP_000580.1). |
| Purirty | Affinity purification |
| Ig Type | Rabbit IgG |
| Formulation | Supplied as a 0.2μm filtered solution in PBS with 0.05% Proclin300, PH 7.4. |
| CrossReactivity | No cross-reactivity in ELISA with recombinant hIL-13、mGM-CSF 、mIL-13、mIL-4、rIL-4、rIL-4 |
| Storage | Store at -20℃. Avoid repeated freeze-thaw cycles. |
Human IL-4 Sanwich Immunoassay
| Recommended Concentration | Sample | |
|---|---|---|
| ELISA Capture | 0.5-2ug/mL | Rabbit anti-Human IL-4(CAP)(Cat. No.RM17735) |
| ELISA Detection | 0.006-0.024ug/mL | Rabbit anti-Human IL-4(DET)(Cat. No.RM17736) |
| Standard | 7.81-500pg/mL | Recombinant Human IL-4 Protein |
