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IL4 Rabbit pAb (A5649)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - IL4 Rabbit pAb (A5649)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with IL4 using IL4 Rabbit pAb(A5649) at 1:1000 dilution. 
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

ABclonal:Immunohistochemistry - IL4 Rabbit pAb (A5649)

Immunohistochemistry analysis of paraffin-embedded Human liver damage using IL4 Rabbit pAb (A5649) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - IL4 Rabbit pAb (A5649)

Immunofluorescence analysis of paraffin-embedded Human breast cancer tissue using IL4 Rabbit pAb (A5649) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameIL4 Rabbit pAb
Catalog No.A5649
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 25-153 of human IL4 (NP_000580.1).
SequenceHKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS
Gene ID
Swiss Prot
SynonymsBSF1; IL-4; BCGF1; BSF-1; BCGF-1; IL4
Calculated MW17kDa
Observed MW20kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T transfected with IL4 protein
Cellular locationSecreted
Customer validation

WB(Mus musculus)

IHC(Rattus norvegicus)

Documents

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ABclonal:Western blot - IL4 Rabbit pAb (A5649)}

Western blot - IL4 Rabbit pAb (A5649)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with IL4 using IL4 Rabbit pAb(A5649) at 1:1000 dilution. 
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.
ABclonal:Immunohistochemistry - IL4 Rabbit pAb (A5649)}

Immunohistochemistry - IL4 Rabbit pAb (A5649)

Immunohistochemistry analysis of paraffin-embedded Human liver damage using IL4 Rabbit pAb (A5649) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - IL4 Rabbit pAb (A5649)}

Immunofluorescence - IL4 Rabbit pAb (A5649)

Immunofluorescence analysis of paraffin-embedded Human breast cancer tissue using IL4 Rabbit pAb (A5649) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A5649? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

재조합 단백질 (1)

ELISA 키트 (2)

제품 (5)

Secondary Antibodies (26)