Recombinant Human IL-4 Protein (RP00995)

Overview

Product name	Recombinant Human IL-4 Protein
Catalog No.	RP00995
Description	Recombinant Human IL-4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His25-Ser153) of human IL-4 (Accession #NP_000580.1) fused with a 6×His tag at the C-terminus.
Bio-Activity	Measured in a cell proliferation assay using TF 1 human erythroleukemic cells. The ED₅₀ for this effect is 85-340 pg/mL, corresponding to a specific activity of 2.94 × 10⁶~1.17× 10⁷ units/mg.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	15.8 kDa

Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype.

Product information

Synonym	BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4; BCGF1; BSF-1; BSF1; IL-4
Expression Host	HEK293 cells
Tag	C-His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.