| Product name | Rabbit anti-Human IgM mAb(DET) |
|---|---|
| Catalog No. | RMK0104 |
| Catagory | Elisa Antibody Kit |
| Application | ELISA |
| Description | Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005). |
| Immunogen | Native Human IgM |
| Purirty | Affinity purification |
| Ig Type | Rabbit IgG |
| Formulation | Supplied as a 0.2um filtered solution in PBS with 0.05%ProClin 300, PH 7.4. |
| CrossReactivity | No cross-reactivity in ELISA assay withrecombinant M.IgM, R.IgM, M.IgE, R.IgA |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Preservative：0.05%ProClin 300. Avoid repeated freeze-thaw cycles. |
Human IgM Sandwich ELISA Immunoassay
| Recommended Concentration | Sample | |
|---|---|---|
| ELISA Capture | 1-4ug/mL | Rabbit anti-Human IgM mAb(CAP)(Cat. No.RMK0103) |
| ELISA Detection | 0.0165-0.066ug/mL | Rabbit anti-Human IgM mAb(DET)(Cat. No.RMK0104) |
| Standard | 0.31-20ng/mL | Recombinant Human IgM Protein |
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