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ABflo® 647 Rabbit anti-Human IgM mAb (A24695)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Immunofluorescence - ABflo® 647 Rabbit anti-Human IgM mAb (A24695)

Confocal imaging of Raji cells using ABflo® 647 Rabbit anti-Human IgM mAb (A24695, dilution 1:100). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - ABflo® 647 Rabbit anti-Human IgM mAb (A24695)

Confocal imaging of Daudi cells using ABflo® 647 Rabbit anti-Human IgM mAb (A24695, dilution 1:100). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human IgM mAb (A24695)

Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and Daudi cells (right) were surface-stained with ABflo® 647 Rabbit anti-Human IgM mAb (A24695, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human IgM mAb (A24695)

Flow cytometry: 1X10^6 Daudi cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human IgM mAb (A24695, 5 μl/Test, right).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human IgM mAb (A24695)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with PE Mouse anti-Human CD19 mAb (A22816, 5 μl/Test) and ABflo® 647 Rabbit anti-Human IgM mAb (A24695, 5 μl/Test, left) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, right).

Overview

Product nameABflo® 647 Rabbit anti-Human IgM mAb
Catalog No.A24695
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC63173-ABflo647
Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010]
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 218-453 of human IgM(P01871).
SequenceQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTGKPTLYNVSLVMSDTAGTCY
Gene ID
Swiss Prot
SynonymsIGHM; AGM1; MU; VH; immunoglobulin heavy constant mu
Calculated MW49kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50-1:200
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationImmunofluorescence    Flow Cytometry    
Positive samples
Cellular locationCell membrane, Secreted, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunofluorescence - ABflo® 647 Rabbit anti-Human IgM mAb (A24695)}

    Immunofluorescence - ABflo® 647 Rabbit anti-Human IgM mAb (A24695)

    Confocal imaging of Raji cells using ABflo® 647 Rabbit anti-Human IgM mAb (A24695, dilution 1:100). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - ABflo® 647 Rabbit anti-Human IgM mAb (A24695)}

    Immunofluorescence - ABflo® 647 Rabbit anti-Human IgM mAb (A24695)

    Confocal imaging of Daudi cells using ABflo® 647 Rabbit anti-Human IgM mAb (A24695, dilution 1:100). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human IgM mAb (A24695)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Human IgM mAb (A24695)

    Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and Daudi cells (right) were surface-stained with ABflo® 647 Rabbit anti-Human IgM mAb (A24695, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human IgM mAb (A24695)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Human IgM mAb (A24695)

    Flow cytometry: 1X10^6 Daudi cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human IgM mAb (A24695, 5 μl/Test, right).
    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human IgM mAb (A24695)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Human IgM mAb (A24695)

    Flow cytometry: 1X10^6 Human PBMC were surface-stained with PE Mouse anti-Human CD19 mAb (A22816, 5 μl/Test) and ABflo® 647 Rabbit anti-Human IgM mAb (A24695, 5 μl/Test, left) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.

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