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Rabbit anti-Human IgM mAb (A24260)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Rabbit anti-Human IgM mAb (A24260)

Western blot analysis of lysates from Raji cells, using Rabbit anti-Human IgM mAb (A24260) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): Jurkat
Exposure time: 20s.

ABclonal:Immunofluorescence - Rabbit anti-Human IgM mAb (A24260)

Confocal imaging of Daudi cells using Rabbit anti-Human IgM mAb (A24260, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - Rabbit anti-Human IgM mAb (A24260)

Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and Daudi cells (right) were surface-stained with Rabbit anti-Human IgM mAb (A24260, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - Rabbit anti-Human IgM mAb (A24260)

Flow cytometry: 1X10^6 Daudi cells were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or Rabbit anti-Human IgM mAb (A24260, 2 μg/mL, right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.

ABclonal:Flow CytoMetry - Rabbit anti-Human IgM mAb (A24260)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or Rabbit anti-Human IgM mAb (A24260, 2 μg/mL, right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.

Overview

Product nameRabbit anti-Human IgM mAb
Catalog No.A24260
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC63173
Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 218-453 of Human IgM(P01871).
SequenceQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTGKPTLYNVSLVMSDTAGTCY
Gene ID
Swiss Prot
SynonymsMU; VH; AGM1; Human IgM
Calculated MW49kDa
Observed MW75kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • IF/ICC 1:200 - 1:800
  • FC 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Flow Cytometry    
Positive samplesRaji
Cellular locationBlood microparticle, Cell surface, External side of plasma membrane, Extracellular exosome, Extracellular space, Immunoglobulin complex, Circulating, Plasma membrane.

Documents

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Lot number

    ABclonal:Western blot - Rabbit anti-Human IgM mAb (A24260)}

    Western blot - Rabbit anti-Human IgM mAb (A24260)

    Western blot analysis of lysates from Raji cells, using Rabbit anti-Human IgM mAb (A24260) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Negative control (NC): Jurkat
    Exposure time: 20s.
    ABclonal:Immunofluorescence - Rabbit anti-Human IgM mAb (A24260)}

    Immunofluorescence - Rabbit anti-Human IgM mAb (A24260)

    Confocal imaging of Daudi cells using Rabbit anti-Human IgM mAb (A24260, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Flow CytoMetry - Rabbit anti-Human IgM mAb (A24260)}

    Flow CytoMetry - Rabbit anti-Human IgM mAb (A24260)

    Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and Daudi cells (right) were surface-stained with Rabbit anti-Human IgM mAb (A24260, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - Rabbit anti-Human IgM mAb (A24260)}

    Flow CytoMetry - Rabbit anti-Human IgM mAb (A24260)

    Flow cytometry: 1X10^6 Daudi cells were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or Rabbit anti-Human IgM mAb (A24260, 2 μg/mL, right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.
    ABclonal:Flow CytoMetry - Rabbit anti-Human IgM mAb (A24260)}

    Flow CytoMetry - Rabbit anti-Human IgM mAb (A24260)

    Flow cytometry: 1X10^6 Human PBMC were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or Rabbit anti-Human IgM mAb (A24260, 2 μg/mL, right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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