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Biotinylated Recombinant Human ALCAM/CD166 Protein (RP00621B)

Datasheet

Overview

Product nameBiotinylated Recombinant Human ALCAM/CD166 Protein
Catalog No.RP00621B
DescriptionBiotinylated Recombinant Human ALCAM/CD166 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Trp28-Ala526) of Human ALCAM/CD166 (Accession #Q13740-1) fused with a C-His&Avi tag at the C-terminus.
Purity> 95% by SDS-PAGE;> 95% by HPLC
Endotoxin< 1 EU/μg of the protein by LAL method
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
SpeciesHuman
Calculated MW58.9 kDa
Brain metastasis (BM) in non-small-cell lung cancer (NSCLC) has a very poor prognosis. Recent studies have demonstrated the importance of cell adhesion molecules in tumor metastasis.Elevated levels of ALCAM expression promote BM formation in NSCLC through increased tumor cell dissemination and interaction with the brain endothelial cells. Therefore, ALCAM could be targeted to reduce the occurrence of BM.
SynonymCD166; ALCAM; MEMD; CD6L; KG-CAM
Expression HostHEK293 cells
TagC-His&Avi
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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