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PE Rabbit anti-Human CD166/ALCAM mAb (A23710)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - PE Rabbit anti-Human CD166/ALCAM mAb (A23710)

Flow cytometry: 1X10^6 K562 cells(negative control, left) and SH-SY5Y(right) cells were surface-stained with PE Rabbit anti-Human CD166/ALCAM mAb(A23710, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (5 μl/Test, blue line).Non-fluorescently stained cells was used as blank control (red line).

ABclonal:Flow CytoMetry - PE Rabbit anti-Human CD166/ALCAM mAb (A23710)

Flow cytometry:1X10^6 SH-SY5Y cells were surface-stained with PE Rabbit IgG isotype control (5 μl/Test, left) or PE Rabbit anti-Human CD166/ALCAM mAb(A23710, 5 μl/Test, right).

Overview

Product namePE Rabbit anti-Human CD166/ALCAM mAb
Catalog No.A23710
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC57933-PE
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 28-526 of human CD166/ALCAM (NP_001618.2).
SequenceWYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDNVFEAPTIVKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVLHPLEGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGPSGQKTIHSEQAVFDIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPPPEEFLFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKKSMIASTAITVHYLDLSLNPSGEVTRQIGDALPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKMTKKTDPSGLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISPEENVTLTCTAENQLERTVNSLNVSAISIPEHDEADEISDENREKVNDQA
Gene ID
Swiss Prot
SynonymsMEMD; CD166
Calculated MW15kDa/33kDa/63kDa/65kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationExternal side of plasma membrane, extracellular exosome, focal adhesion, immunological synapse, plasma membrane

Documents

Certificate of Compliance

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ABclonal:Flow CytoMetry - PE Rabbit anti-Human CD166/ALCAM mAb (A23710)}

Flow CytoMetry - PE Rabbit anti-Human CD166/ALCAM mAb (A23710)

Flow cytometry: 1X10^6 K562 cells(negative control, left) and SH-SY5Y(right) cells were surface-stained with PE Rabbit anti-Human CD166/ALCAM mAb(A23710, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (5 μl/Test, blue line).Non-fluorescently stained cells was used as blank control (red line).
ABclonal:Flow CytoMetry - PE Rabbit anti-Human CD166/ALCAM mAb (A23710)}

Flow CytoMetry - PE Rabbit anti-Human CD166/ALCAM mAb (A23710)

Flow cytometry:1X10^6 SH-SY5Y cells were surface-stained with PE Rabbit IgG isotype control (5 μl/Test, left) or PE Rabbit anti-Human CD166/ALCAM mAb(A23710, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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