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ABflo® 594 Rabbit anti-Human CD166/ALCAM mAb (A24582)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD166/ALCAM mAb (A24582)

Flow cytometry:1X10^6 K-562 cells (negative control, left) and SH-SY5Y cells (right) were surface-stained with ABflo® 594 Rabbit anti-Human CD166/ALCAM mAb(A24582, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD166/ALCAM mAb (A24582)

Flow cytometry:1X10^6 SH-SY5Y cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD166/ALCAM mAb(A24582, 5 μl/Test, right).

Overview

Product nameABflo® 594 Rabbit anti-Human CD166/ALCAM mAb
Catalog No.A24582
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC57933-ABflo594
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 28-526 of human CD166/ALCAM(NP_001618.2)
SequenceWYTVNSAYGD TIIIPCRLDV PQNLMFGKWK YEKPDGSPVF IAFRSSTKKS VQYDDVPEYK DRLNLSENYT LSISNARISD EKRFVCMLVT EDNVFEAPTI VKVFKQPSKP EIVSKALFLE TEQLKKLGDC ISEDSYPDGN ITWYRNGKVL HPLEGAVVII FKKEMDPVTQ LYTMTSTLEY KTTKADIQMP FTCSVTYYGP SGQKTIHSEQ AVFDIYYPTE QVTIQVLPPK NAIKEGDNIT LKCLGNGNPP PEEFLFYLPG QPEGIRSSNT YTLTDVRRNA TGDYKCSLID KKSMIASTAI TVHYLDLSLN PSGEVTRQIG DALPVSCTIS ASRNATVVWM KDNIRLRSSP SFSSLHYQDA GNYVCETALQ EVEGLKKRES LTLIVEGKPQ IKMTKKTDPS GLSKTIICHV EGFPKPAIQW TITGSGSVIN QTEESPYING RYYSKIIISP EENVTLTCTA ENQLERTVNS LNVSAISIPE HDEADEISDE NREKVNDQA
Gene ID
Swiss Prot
SynonymsALCAM; CD166; MEMD; CD166 antigen
Calculated MW15kDa/33kDa/63kDa/65kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationMembrane, Secreted, Single-pass type I membrane protein

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ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD166/ALCAM mAb (A24582)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD166/ALCAM mAb (A24582)

Flow cytometry:1X10^6 K-562 cells (negative control, left) and SH-SY5Y cells (right) were surface-stained with ABflo® 594 Rabbit anti-Human CD166/ALCAM mAb(A24582, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD166/ALCAM mAb (A24582)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD166/ALCAM mAb (A24582)

Flow cytometry:1X10^6 SH-SY5Y cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD166/ALCAM mAb(A24582, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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