Recombinant Human ALCAM/CD166 Protein (RP00119)

Overview

Product name	Recombinant Human ALCAM/CD166 Protein
Catalog No.	RP00119
Description	Recombinant Human ALCAM/CD166 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met1-Ala526) of human ALCAM/CD166 (Accession #NP_001618.2) fused with an Fc, 6×His tag at the C-terminus.
Bio-Activity	1.Measured by its binding ability in a functional ELISA.Immobilized Human CD6 at 1 μg/mL (100 μL/well) can bind Human CD166 with a linear range of 0.05-1.2 ng/mL. 2.Measured by its ability to block adhesion of Jurkat cells to immobilized Recombinant Human CD6 Protein . The ED₅₀ for this effect is 2.7-10.8 μg/mL.
Purity	> 97% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	85.57 kDa

This protein is activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

Product information

Synonym	ALCAM; CD166; MEMD; MEMD
Expression Host	HEK293 cells
Tag	C-hFc&His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.