Biotinylated Recombinant Human B7-H3/CD276 Protein (RP00637B)

Overview

Product name	Biotinylated Recombinant Human B7-H3/CD276 Protein
Catalog No.	RP00637B
Description	Biotinylated Recombinant Human B7-H3/CD276 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu29-Pro245) of Human B7-H3/CD276 (Accession #Q5ZPR3-2) fused with a C-His&Avi tag at the C-terminus.
Purity	> 95% by SDS-PAGE；> 95% by HPLC
Endotoxin	< 1 EU/μg of the protein by LAL method
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Species	Human
Calculated MW	26.49 kDa

B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig?B7?H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.

Product information

Synonym	B7H3; B7-H3; CD276; PSEC0249; UNQ309; PRO352; B7 homolog 3; CD276
Expression Host	HEK293 cells
Tag	C-His&Avi
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.