Recombinant Human B7-H3/CD276 Protein (RP01020)

Product name	Recombinant Human B7-H3/CD276 Protein
Catalog No.	RP01020
Description	Recombinant Human B7-H3/CD276 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu29-Pro245) of human B7-H3 (Accession #NP_079516) fused with a 6×His tag at the C-terminus.
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized Human B7-H3 at 2 μg/mL (100 μL/well) can bind Anti-Human B7-H3 Antibody with a linear range of 1-4 ng/mL.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	24.15 kDa

Synonym	CD276; 4Ig-B7-H3; B7-H3; B7H3; B7RP-2; CD276 antigen; 4Ig-B7-H3; B7-H3; B7H3; B7RP-2
Expression Host	HEK293 cells
Tag	C-His
Swiss-Prot
Gene ID

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.