Biotinylated Recombinant Human Fc-gamma RIII beta/FCGR3B/CD16b Protein (RP00471B)

Overview

Product name	Biotinylated Recombinant Human Fc-gamma RIII beta/FCGR3B/CD16b Protein
Catalog No.	RP00471B
Description	Biotinylated Recombinant Human Fc-gamma RIII beta/FCGR3B/CD16b Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly17-Ser200) of Human Fc-gamma RIII beta/FCGR3B/CD16b (Accession #O75015-1) fused with a C-His&Avi tag at the C-terminus.
Purity	> 95% by SDS-PAGE；> 95% by HPLC
Endotoxin	< 1 EU/μg of the protein by LAL method
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Species	Human
Calculated MW	23.7 kDa

Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.

Product information

Synonym	CD16; Fc gamma RIIIB; FCG3; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10
Expression Host	HEK293 cells
Tag	C-His&Avi
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.