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ABflo® 647 Rabbit anti-Human CD16b mAb (A23402)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD16b mAb (A23402)

Flow cytometry:1X10^6 293F cells(negative control, left) and 293F(Transfection, right) cells were surface-stained with ABflo® 647 Rabbit anti-Human CD16b mAb(A23402, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line).Non-fluorescently stained cells was used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD16b mAb (A23402)

Flow cytometry:1X10^6 293F(Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD16b mAb(A23402, 5 μl/Test, right).

Overview

Product nameABflo® 647 Rabbit anti-Human CD16b mAb
Catalog No.A23402
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC59988-ABf647
This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other responses, including antibody dependent cellular mediated cytotoxicity and antibody dependent enhancement of virus infections. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene are associated with immunodeficiency 20, and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-208 of human CD16b (NP_000561.3).
SequenceTEDLPKAVVFLEPQWYSVLEKDSVTLKCQGAYSPEDNSTQWFHNESLISSQASSYFIDAATVNDSGEYRCQTNLSTLSDPVQLEVHIGWLLLQAPRWVFKEEDPIHLRCHSWKNTALHKVTYLQNGKDRKYFHHNSDFHIPKATLKDSGSYFCRGLVGSKNVSSETVNITITQGLAVSTISSFSPPGYQ
Gene ID
Swiss Prot
SynonymsCD16; CD16-I; CD16b; FCG3; FCGR3; FCGR3B; FCR-10; FCRIII; FCRIIIb
Calculated MW26KDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationExtracellular exosome, extracellular region, plasma membrane

Documents

Certificate of Compliance

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ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD16b mAb (A23402)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD16b mAb (A23402)

Flow cytometry:1X10^6 293F cells(negative control, left) and 293F(Transfection, right) cells were surface-stained with ABflo® 647 Rabbit anti-Human CD16b mAb(A23402, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line).Non-fluorescently stained cells was used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD16b mAb (A23402)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD16b mAb (A23402)

Flow cytometry:1X10^6 293F(Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD16b mAb(A23402, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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