Overview
Product name | Recombinant Human Fc-gamma RIII beta/FCGR3B/CD16b Protein |
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Catalog No. | RP00291 |
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Description | Recombinant Human Fc-gamma RIII beta/FCGR3B/CD16b Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr20-Gln208) of human Fc gamma RIIIB/CD16b (Accession #NP_000561.3) fused with a 6×His tag at the C-terminus. |
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Bio-Activity | Measured by its binding ability in a functional ELISA.Immobilized APC anti-human CD16 Antibody at 1μg/mL (25 μL/well) can bind Human FCGR3B with a linear range of 0.46-1.16ng/mL.
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Purity | > 97% by SDS-PAGE. |
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Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
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Species | Human |
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Calculated MW | 22.21 kDa |
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Background
CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors function in the activation or inhibition of immune responses. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16B is also kown as FCGR3B and FCG3B, is expressed specifically by polymorphonuclear leukocytes (neutrophils) and stimulated eosinophils. CD16B is the low affinity receptor for the Fc region of immunoglobulins gamma. CD16B may serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Product information
Synonym | FCGR3B; CD16; CD16b; FCG3; FCGR3; FCR-10; FCRIII; FCRIIIb |
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Expression Host | HEK293 cells |
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Tag | C-His |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.