Biotinylated Recombinant Human Glypican-3/GPC3 Protein (RP02371B)

Overview

Product name	Biotinylated Recombinant Human Glypican-3/GPC3 Protein
Catalog No.	RP02371B
Description	Biotinylated Recombinant Human Glypican-3/GPC3 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gln25-His559) of Human GPC3 fused with a His tag and Avi tag at the C-terminal.
Purity	> 95% by Tris-Bis PAGE
Endotoxin	< 1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Species	Human
Calculated MW	63.6 kDa

Glypican-3 is a protein , which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.

Product information

Synonym	GPC3; DGSX; GTR2-2; MXR7; OCI-5; SDYS; SGB; SGBS; SGBS1; GTR2-2; MXR7; OCI-5; SDYS; SGB; SGBS; SGBS1; glypican-3
Expression Host	HEK293 cells
Tag	C-His&Avi
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.