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APC Rabbit anti-Human Glypican 3 (GPC3) mAb (A26131)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - APC Rabbit anti-Human Glypican 3 (GPC3) mAb (A26131)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and Hep G2 cells (right) were surface-stained with APC Rabbit anti-Human Glypican 3 (GPC3) mAb (A26131, 5 μl/Test, orange line) or APC Rabbit IgG isotype control (A24173, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - APC Rabbit anti-Human Glypican 3 (GPC3) mAb (A26131)

Flow cytometry: 1X10^6 Hep G2 cells were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human Glypican 3 (GPC3) mAb (A26131, 5 μl/Test, right).

Overview

Product nameAPC Rabbit anti-Human Glypican 3 (GPC3) mAb
Catalog No.A26131
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC56916-APC
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 481-580 of human Glypican 3 (GPC3) (NP_004475.1).
SequenceGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEISTFHNLGNVHSPLKLLTSMAISVVCFFFLVH
Gene ID
Swiss Prot
SynonymsSGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
Calculated MW66kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Lipid-anchor, GPI-anchor, cell surface, endoplasmic reticulum lumen, Golgi lumen, lysosomal lumen, plasma membrane, Extracellular side, GPI-anchor, Secreted, extracellular space.

Documents

Certificate of Compliance

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ABclonal:Flow CytoMetry - APC Rabbit anti-Human Glypican 3 (GPC3) mAb (A26131)}

Flow CytoMetry - APC Rabbit anti-Human Glypican 3 (GPC3) mAb (A26131)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and Hep G2 cells (right) were surface-stained with APC Rabbit anti-Human Glypican 3 (GPC3) mAb (A26131, 5 μl/Test, orange line) or APC Rabbit IgG isotype control (A24173, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - APC Rabbit anti-Human Glypican 3 (GPC3) mAb (A26131)}

Flow CytoMetry - APC Rabbit anti-Human Glypican 3 (GPC3) mAb (A26131)

Flow cytometry: 1X10^6 Hep G2 cells were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human Glypican 3 (GPC3) mAb (A26131, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (6)

재조합 단백질 (2)

Secondary Antibodies (26)