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Recombinant Human Argonaute-2/AGO2 Protein (RP01132)

Publication (1) Datasheet

Overview

Product nameRecombinant Human Argonaute-2/AGO2 Protein
Catalog No.RP01132
DescriptionRecombinant Human Argonaute-2/AGO2 Protein is produced by Insect cell-baculovirus expression system. The target protein is expressed with sequence (Met1-Ala859) of Human Argonaute-2/AGO2 (Accession #NP_036286.2) fused with a 6×His tag at the N-terminus.
Purity> 85 % by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution 20mM Tris, 500mM NaCl, pH7.4, 10% glycerol, 2mM DTT.Contact us for customized product form or formulation.
SpeciesHuman
Calculated MW99 kDa
This protein is a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.
SynonymAGO2; CASC7; EIF2C2; LINC00980; PPD; Q10; protein argonaute-2; Argonaute 2; EIF2C2
Expression HostBaculovirus-Infected Sf9 Cells
TagN-6×His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using RP01132? Please let us know so that we can cite the reference in this datasheet.

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