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[KD Validated] Argonaute-2 Rabbit mAb (A25642)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Western blot analysis of lysates from wild type (WT) and Argonaute-2 knockdown (KD) HeLa cells using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at 1:2900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Western blot analysis of various lysates using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at 1:2900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at a dilution of  1:600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at a dilution of  1:600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at a dilution of  1:600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at a dilution of  1:600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Confocal imaging of Hep G2 cells using [KD Validated] Argonaute-2 Rabbit mAb (A25642, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Confocal imaging of NIH/3T3 cells using [KD Validated] Argonaute-2 Rabbit mAb (A25642, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Confocal imaging of PC-12 cells using [KD Validated] Argonaute-2 Rabbit mAb (A25642, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name[KD Validated] Argonaute-2 Rabbit mAb
Catalog No.A25642
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC66928
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 101-200 of human Argonaute-2 (NP_036286.2).
SequenceYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWF
Gene ID
Swiss Prot
SynonymsPPD; Q10; CASC7; EIF2C2; LESKRES; LINC00980
Calculated MW97kDa
Observed MW96kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:8000
  • IHC-P 1:600 - 1:6000
  • IF/ICC 1:300 - 1:1200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, MCF-7, Mouse kidney
Cellular locationCytoplasm, Nucleus.

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Certificate of Compliance

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ABclonal:Western blot - [KD Validated] Argonaute-2 Rabbit mAb (A25642)}

Western blot - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Western blot analysis of lysates from wild type (WT) and Argonaute-2 knockdown (KD) HeLa cells using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at 1:2900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KD Validated] Argonaute-2 Rabbit mAb (A25642)}

Western blot - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Western blot analysis of various lysates using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at 1:2900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KD Validated] Argonaute-2 Rabbit mAb (A25642)}

Immunohistochemistry - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at a dilution of  1:600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] Argonaute-2 Rabbit mAb (A25642)}

Immunohistochemistry - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at a dilution of  1:600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] Argonaute-2 Rabbit mAb (A25642)}

Immunohistochemistry - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at a dilution of  1:600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] Argonaute-2 Rabbit mAb (A25642)}

Immunohistochemistry - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at a dilution of  1:600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KD Validated] Argonaute-2 Rabbit mAb (A25642)}

Immunofluorescence - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Confocal imaging of Hep G2 cells using [KD Validated] Argonaute-2 Rabbit mAb (A25642, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - [KD Validated] Argonaute-2 Rabbit mAb (A25642)}

Immunofluorescence - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Confocal imaging of NIH/3T3 cells using [KD Validated] Argonaute-2 Rabbit mAb (A25642, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - [KD Validated] Argonaute-2 Rabbit mAb (A25642)}

Immunofluorescence - [KD Validated] Argonaute-2 Rabbit mAb (A25642)

Confocal imaging of PC-12 cells using [KD Validated] Argonaute-2 Rabbit mAb (A25642, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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