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Argonaute-2 Rabbit pAb (A6023)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Argonaute-2 Rabbit pAb (A6023)

Western blot analysis of various lysates using Argonaute-2 Rabbit pAb (A6023) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Argonaute-2 Rabbit pAb (A6023)

Immunohistochemistry analysis of paraffin-embedded Human stomach using Argonaute-2 Rabbit pAb (A6023) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Argonaute-2 Rabbit pAb (A6023)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using Argonaute-2 Rabbit pAb (A6023) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Argonaute-2 Rabbit pAb (A6023)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using Argonaute-2 Rabbit pAb (A6023) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameArgonaute-2 Rabbit pAb
Catalog No.A6023
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Argonaute-2 (NP_036286.2).
SequenceMYSGAGPALAPPAPPPPIQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNL
Gene ID
Swiss Prot
SynonymsPPD; Q10; CASC7; EIF2C2; LESKRES; LINC00980; Argonaute-2
Calculated MW97kDa
Observed MW97kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse heart, Mouse brain, Rat brain, Rat heart
Cellular locationCytoplasm, Nucleus, P-body
Customer validation

ChIP(Sus scrofa)

Documents

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ABclonal:Western blot - Argonaute-2 Rabbit pAb (A6023)}

Western blot - Argonaute-2 Rabbit pAb (A6023)

Western blot analysis of various lysates using Argonaute-2 Rabbit pAb (A6023) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Argonaute-2 Rabbit pAb (A6023)}

Immunohistochemistry - Argonaute-2 Rabbit pAb (A6023)

Immunohistochemistry analysis of paraffin-embedded Human stomach using Argonaute-2 Rabbit pAb (A6023) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Argonaute-2 Rabbit pAb (A6023)}

Immunohistochemistry - Argonaute-2 Rabbit pAb (A6023)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using Argonaute-2 Rabbit pAb (A6023) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Argonaute-2 Rabbit pAb (A6023)}

Immunohistochemistry - Argonaute-2 Rabbit pAb (A6023)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using Argonaute-2 Rabbit pAb (A6023) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A6023? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

재조합 단백질 (1)

Secondary Antibodies (26)