Overview
Product name | Recombinant Human Caspase-3/CASP3 Protein |
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Catalog No. | RP02120 |
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Description | Recombinant Human Caspase-3/CASP3 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser29-His277) of human Caspase-3/CASP3 (Accession #NP_001012435.1) fused with a 6×His tag at the C-terminus. |
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Purity | ≥ 95 % as determined by SDS-PAGE |
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Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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Species | Human |
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Product information
Synonym | CASP3; CPP32; Caspase-3; CASP-3; EC:3.4.22.56; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12 |
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Expression Host | E. coli |
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Tag | C-6His |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.