제품 > 항체 > 다중클론항체(pAb)

Caspase-3 Rabbit pAb (A2156)

Reviews (6)Publications (240) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Caspase-3 Rabbit pAb
ABclonal:Western blot - Caspase-3 Rabbit pAb (A2156)

Western blot analysis of various lysates using [KO Validated] Caspase-3 Rabbit pAb (A2156) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Caspase-3 Rabbit pAb (A2156)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using [KO Validated] Caspase-3 Rabbit pAb (A2156) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Caspase-3 Rabbit pAb (A2156)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using [KO Validated] Caspase-3 p17 Rabbit pAb (A2156) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Caspase-3 Rabbit pAb (A2156)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using [KO Validated] Caspase-3 p17 Rabbit pAb (A2156) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Caspase-3 Rabbit pAb (A2156)

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Caspase-3 Rabbit pAb (A2156) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Caspase-3 Rabbit pAb (A2156)

Immunofluorescence analysis of PC-12 cells using [KO Validated] Caspase-3 Rabbit pAb (A2156) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Caspase-3 Rabbit pAb (A2156)

Immunofluorescence analysis of PC-12 cells using [KO Validated] Caspase-3 Rabbit pAb (A2156) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Caspase-3 Rabbit pAb (A2156)

Immunofluorescence analysis of U2OS cells using [KO Validated] Caspase-3 Rabbit pAb (A2156) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Caspase-3 Rabbit pAb(A2156)
Review
ABclonal: review for Caspase-3 Rabbit pAb(A2156)
Review
ABclonal: review for Caspase-3 Rabbit pAb(A2156)
Review
ABclonal: review for Caspase-3 Rabbit pAb(A2156)
Review
ABclonal: review for Caspase-3 Rabbit pAb(A2156)
Review
ABclonal: review for Caspase-3 Rabbit pAb(A2156)

Overview

Product nameCaspase-3 Rabbit pAb
Catalog No.A2156
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human Caspase-3 (NP_004337.2).
SequenceFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFII
Gene ID
Swiss Prot
SynonymsCPP32; SCA-1; CPP32B; -3
Calculated MW32kDa
Observed MW32kDa, 17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, Mouse lung
Cellular locationCytoplasm.
Customer validation

(HeLa, Mouse kidney, Rat brain, Human uterine stromal cells, B6, NMuMG)

WB(Homo sapiens, Sus scrofa, Mus musculus, Gallus gallus, Bos taurus, Rattus norvegicus, Spodoptera frugiperda, Ctenopharyngodon, Ctenopharyngodon idellus, quails, Piglet, Sus domesticus, Capra hircus, Other, Mus musculus, ovis aries, Rattus norvegicus, Oryctolagus cuniculus, Lampetra japonica)

IHC(Mus musculus, Actinopterygii, Danio rerio, Rattus norvegicus, Homo sapiens, Canis familiaris)

IHC(Mus musculus, Homo sapiens, Rattus norvegicus)

IF(Homo sapiens, Mus musculus, Rattus norvegicus)

IF(Gallus gallus, Mus musculus)

Co-IP(Mus musculus)

FC(Mus musculus)

WB(Homo sapiens)

Other(Mus musculus)

qPCR(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Caspase-3 Rabbit pAb (A2156)}

Western blot - Caspase-3 Rabbit pAb (A2156)

Western blot analysis of various lysates using [KO Validated] Caspase-3 Rabbit pAb (A2156) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Caspase-3 Rabbit pAb (A2156)}

Immunohistochemistry - Caspase-3 Rabbit pAb (A2156)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using [KO Validated] Caspase-3 Rabbit pAb (A2156) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Caspase-3 Rabbit pAb (A2156)}

Immunohistochemistry - Caspase-3 Rabbit pAb (A2156)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using [KO Validated] Caspase-3 p17 Rabbit pAb (A2156) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Caspase-3 Rabbit pAb (A2156)}

Immunohistochemistry - Caspase-3 Rabbit pAb (A2156)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using [KO Validated] Caspase-3 p17 Rabbit pAb (A2156) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Caspase-3 Rabbit pAb (A2156)}

Immunofluorescence - Caspase-3 Rabbit pAb (A2156)

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Caspase-3 Rabbit pAb (A2156) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Caspase-3 Rabbit pAb (A2156)}

Immunofluorescence - Caspase-3 Rabbit pAb (A2156)

Immunofluorescence analysis of PC-12 cells using [KO Validated] Caspase-3 Rabbit pAb (A2156) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Caspase-3 Rabbit pAb (A2156)}

Immunofluorescence - Caspase-3 Rabbit pAb (A2156)

Immunofluorescence analysis of PC-12 cells using [KO Validated] Caspase-3 Rabbit pAb (A2156) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Caspase-3 Rabbit pAb (A2156)}

Immunofluorescence - Caspase-3 Rabbit pAb (A2156)

Immunofluorescence analysis of U2OS cells using [KO Validated] Caspase-3 Rabbit pAb (A2156) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A2156

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Publishing research using A2156? Please let us know so that we can cite the reference in this datasheet.

항체 (6)

ELISA 키트 (1)

Secondary Antibodies (26)