| Product name | Recombinant Human IFN-alpha 2 Protein |
|---|---|
| Catalog No. | RP01432 |
| Description | Recombinant Human IFN-alpha 2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys24-Glu188) of human Interferon alpha 2/IFNA2 (Accession #NP_000596.2) fused with a 6×His tag at the C-terminus. |
| Bio-Activity | 1.Measured by its binding ability in a functional ELISA.Immobilized Human IFNA2 at 2 μg/mL (100 μL/well) can bind Human IFNAR2 with a linear range of 0.2-617ng/mL. 2.Recombinant human IFN-α2 (10 ng/mL) was used to treat HCT116 cells. Western-blot result showed that both the level of phosphorylated STAT1 and STAT3 were increased, indicating the stimulation was successful.(Customer Feedback Data) |
| Purity | > 95% by SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Species | Human |
| Calculated MW | 20.08 kDa |
| Synonym | IFNA2; IFN-alphaA; IFNA; IFNA2B; INFA2; interferon alpha-2; Interferon alpha 2 (IFN-α2）; IFN-alphaA; IFNA; IFNA2B; INFA2 |
|---|---|
| Expression Host | HEK293 cells |
| Tag | C-His |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
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