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Interferon alpha 2 (IFN-α2） Rabbit mAb (A21263)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with IFN-α2 using Interferon alpha 2 (IFN-α2） Rabbit mAb (A21263) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

ABclonal:Immunohistochemistry - Interferon alpha 2 (IFN-α2） Rabbit mAb (A21263)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Interferon alpha 2 (IFN-α2） Rabbit mAb (A21263) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse thymus using Interferon alpha 2 (IFN-α2） Rabbit mAb (A21263) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat lung using Interferon alpha 2 (IFN-α2） Rabbit mAb (A21263) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product name	Interferon alpha 2 (IFN-α2） Rabbit mAb
Catalog No.	A21263
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC53291

Background

This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded cytokine is a member of the type I interferon family that is produced in response to viral infection as a key part of the innate immune response with potent antiviral, antiproliferative and immunomodulatory properties. This cytokine, like other type I interferons, binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. The encoded protein is effective in reducing the symptoms and duration of the common cold and in treating many types of cancer, including some hematological malignancies and solid tumors. A deficiency of type I interferon in the blood is thought to be a hallmark of severe COVID-19 and may provide a rationale for a combined therapeutic approach.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 24-188 of human Interferon alpha 2 (IFN-α2） (NP_000596.2).
Sequence	CDLPQTHSLGSRRTLMLLAQMRRISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE
Gene ID
Swiss Prot
Synonyms	IFNA; IFNA2B; leIF A; IFN-alphaA; IFN-alpha-2; Interferon alpha 2 (IFN-α2）
Calculated MW	22kDa
Observed MW	20kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:6000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	293T transfected with IFN-α2(Human)
Cellular location	Extracellular region, Extracellular space
Customer validation	IHC(Mus musculus)