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Recombinant Human IgA1 Protein (RPT0023)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Human IgA1 Protein
Catalog No.RPT0023
DescriptionRecombinant Recombinant Human IgA1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys122-Tyr353) of Human IgA1 (Accession #P01876-1) fused with His tag at the N-terminus.
Bio-ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human IGHA1 (Catalog: RPT0023) at 5 μg/mL (100 μL/well) can bind Human CD89/FCAR (Catalog: RP00113) with a linear range of 0.01-7.4 ng/mL.
Purity> 95% by SDS-PAGE.
Endotoxin<1EU/μg of the protein by LAL method
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
SpeciesHuman
Calculated MW26.27 kDa
Recombinant Human IgA1 Protein ,Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens . The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen .
SynonymImmunoglobulin heavy constant alpha 1; Ig alpha-1 chain C region; Ig alpha-1 chain C region BUR; Ig alpha-1 chain C region TRO ; IGHA1 ; IgA1
Expression HostHEK293 cells
TagN-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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