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Human IgA1 Rabbit mAb (A23542)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Human IgA1 Rabbit mAb (A23542)

Western blot analysis of various lysates, using Human IgA1 Rabbit mAb (A23542) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameHuman IgA1 Rabbit mAb
Catalog No.A23542
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60394
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 99-353 of human IgA1. (P01876).
SequenceVPCPVPSTPPTPSPSTPPTPSPSCCHPRLSLHRPALEDLLLGSEANLTCTLTGLRDASGVTFTWTPSSGKSAVQGPPERDLCGCYSVSSVLPGCAEPWNHGKTFTCTAAYPESKTPLTATLSKSGNTFRPEVHLLPPPSEELALNELVTLTCLARGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQGTTTFAVTSILRVAAEDWKKGDTFSCMVGHEALPLAFTQKTIDRLADWQMPPPYVVLDLPQETLEE
Gene ID
Swiss Prot
SynonymsIgA1
Calculated MW37KDa/42KDa
Observed MW60kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHuman plasma
Cellular locationsecreted, Cell membrane, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Human IgA1 Rabbit mAb (A23542)}

    Western blot - Human IgA1 Rabbit mAb (A23542)

    Western blot analysis of various lysates, using Human IgA1 Rabbit mAb (A23542) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    ELISA 키트 (1)

    항체 (5)

    재조합 단백질 (1)

    Secondary Antibodies (26)