Overview
| Product name | Recombinant Human Mature BMP-4 Protein |
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| Catalog No. | RP02512S1 |
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| Description | Recombinant Human Mature BMP-4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser293-Arg408) of Human Mature BMP-4 (Accession #NP_001193.2) fused with no tag. |
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| Bio-Activity | Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is 5.88-23.52 ng/mL, corresponding to a specific activity of 4.25×104~1.70×105 units/mg.
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| Purity | > 97 % as determined by SDS-PAGE |
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| Endotoxin | < 0.01 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of 50mM acetic acid, pH3.6. |
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| Species | Human |
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| Calculated MW | 13.13 kDa |
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Background
Recombinant Human Mature BMP-4 Protein that in humans is encoded by BMP4 gene. BMP4 is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. BMP4 is highly conserved evolutionarily. BMP4 is found in early embryonic development in the ventral marginal zone and in the eye, heart blood and otic vesicle.
Product information
| Synonym | BMP4; BMP2B; DVR4; Bone morphogenetic protein 4; BMP-4; Bone morphogenetic protein 2B; BMP-2B |
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| Expression Host | HEK293 cells |
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| Tag | No-Tag |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
