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Recombinant Human/Mouse/Rat mature BMP-2 Protein (RP02510S)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Human/Mouse/Rat mature BMP-2 Protein
Catalog No.RP02510S
DescriptionRecombinant Human/Mouse/Rat mature BMP-2 Protein is produced by Escherichia coli expression system. The target protein is expressed with sequence (Ala284-Arg396) of Human/Mouse/Rat BMP-2 (NP_001191.1) fused with no tag.
Bio-ActivityMeasured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is 24.59-98.36 ng/mL, corresponding to a specific activity of 1.02×104~4.07×104 units/mg.
Purity> 95% by SDS-PAGE.
Endotoxin< 1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of 0.1% TFA, 30% ACN. Contact us for customized product form or formulation.
SpeciesHuman
Calculated MW12.78 kDa
BMP-2 like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It is also involved in cardiac cell differentiation and epithelial to mesenchymal transition. Like many other proteins from the BMP family, BMP-2 has been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2 may be involved in white adipogenesis and may have metabolic effects.
SynonymBDA2; BMP2A; BMP2
Expression HostE.coli
TagNo tag
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionReconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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