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PE Rabbit anti-Human Phospho-Histone H2AX-S139 mAb (AP1529)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 HeLa cells (negative control, left) and HeLa cells (treated with UV for 30min, right) were intracellularly-stained with PE Rabbit anti-Human Phospho-Histone H2AX-S139 mAb (AP1529, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and NIH/3T3 cells (treated with UV for 30min, right) were intracellularly-stained with PE Rabbit anti-Human Phospho-Histone H2AX-S139 mAb (AP1529, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Overview

Product name	PE Rabbit anti-Human Phospho-Histone H2AX-S139 mAb
Catalog No.	AP1529
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC70320-PE

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S139 of human Histone H2AX (NP_002096.1).
Sequence	QASQEY
Gene ID
Swiss Prot
Synonyms	H2A.X; H2A/X; H2AFX
Calculated MW	15kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC (intra) 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application
Positive samples
Cellular location	Chromosome, Nucleus.