Biotinylated Recombinant Human TNF-alpha Protein (RP02081B)

Overview

Product name	Biotinylated Recombinant Human TNF-alpha Protein
Catalog No.	RP02081B
Description	Biotinylated Recombinant Human TNF-alpha Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Val77-Leu233 ) of Human TNF-alpha fused with a His and Avi tag at the C-terminal.
Purity	> 95% by Tris-Bis PAGE;> 95% by SEC-HPLC
Endotoxin	< 1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Species	Human
Calculated MW	20.3 kDa

Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Product information

Synonym	TNF; DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F; tumor necrosis factor; TNF-α; DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F; TNF alpha
Expression Host	HEK293 cells
Tag	C-His&Avi
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.