Recombinant Human TNF-alpha Protein (RP00993)

Overview

Product name	Recombinant Human TNF-alpha Protein
Catalog No.	RP00993
Description	Recombinant Human TNF-alpha Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val 77 - Leu 233 ) of human TNF-alpha (Accession #NP_000585.2) fused with a 6×His tag at the C-terminus.
Bio-Activity	1.Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is typically 3.46-13.84 pg/mL, corresponding to a specific activity of 7.23×10⁷~2.89×10⁸ units/mg. 2.Recombinant human TNF-α (20 ng/mL) was used to treat HeLa cells. Western-blot result showed that the level of phosphorylated p65 was increased within 10 min, and then decreased rapidly, indicating that the NF-κB was activated successfully.(Customer Feedback Data)
Purity	> 95% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	18.19 kDa

Tumor necrosis factor alpha (TNF-alpha), also kNown as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. KNockout studies in mice also suggested the neuroprotective function of this cytokine.

Product information

Synonym	TNF; DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F; tumor necrosis factor; TNF-α; DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F; TNF alpha
Expression Host	HEK293 cells
Tag	C-His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.