Overview
Product name | FITC-Labeled Recombinant Human HER1/ERBB1/EGFR Protein |
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Catalog No. | RP00498F |
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Description | FITC-Labeled Recombinant Human ERBB1/HER1/EGFR Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu25-Ser645) of Human ERBB1/HER1/EGFR (Accession #P00533-1) fused with a C-His&Avi tag at the C-terminus. |
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Purity | > 95% by SDS-PAGE;> 95% by HPLC |
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Endotoxin | < 1 EU/μg of the protein by LAL method |
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Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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Species | Human |
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Calculated MW | 71.5 kDa |
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Background
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Product information
Synonym | ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2 |
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Expression Host | HEK293 cells |
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Tag | C-His&Avi |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.