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Phospho-EGFR-Y1086 Rabbit pAb (AP0820)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-EGFR-Y1086 Rabbit pAb (AP0820)

Western blot analysis of lysates from A-431 cells, using Phospho-EGFR-Y1086 Rabbit pAb (AP0820) at 1:1000 dilution or EGFR antibody (A11351). A-431 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product namePhospho-EGFR-Y1086 Rabbit pAb
Catalog No.AP0820
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
ImmunogenA synthetic phosphorylated peptide around Y1086 of human EGFR (NP_005219.2).
SequencePVYHN
Gene ID
Swiss Prot
SynonymsERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2; Phospho-EGFR-Y1086
Calculated MW134kDa
Observed MW175kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-431 treated by EGF
Cellular locationCell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens)

Co-IP(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - Phospho-EGFR-Y1086 Rabbit pAb (AP0820)}

Western blot - Phospho-EGFR-Y1086 Rabbit pAb (AP0820)

Western blot analysis of lysates from A-431 cells, using Phospho-EGFR-Y1086 Rabbit pAb (AP0820) at 1:1000 dilution or EGFR antibody (A11351). A-431 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using AP0820? Please let us know so that we can cite the reference in this datasheet.

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Secondary Antibodies (26)