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[KD Validated] EGFR Rabbit pAb (A11577)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from wild type(WT) and EGFR knockdown (KD) HeLa cells, using [KD Validated] EGFR Rabbit pAb (A11577) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

ABclonal:Immunohistochemistry - [KD Validated] EGFR Rabbit pAb (A11577)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using [KD Validated] EGFR Rabbit pAb (A11577) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human placenta using [KD Validated] EGFR Rabbit pAb (A11577) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KD Validated] EGFR Rabbit pAb (A11577)

Immunofluorescence analysis of A-431 cells using [KD Validated] EGFR Rabbit pAb (A11577) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	[KD Validated] EGFR Rabbit pAb
Catalog No.	A11577
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
Sequence	QGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA
Gene ID
Swiss Prot
Synonyms	ERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2; FR
Calculated MW	134kDa
Observed MW	175kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa
Cellular location	Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein
Customer validation	WB(Homo sapiens, Mus musculus) IP(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KD Validated] EGFR Rabbit pAb images

Western blot - [KD Validated] EGFR Rabbit pAb (A11577)

Immunohistochemistry - [KD Validated] EGFR Rabbit pAb (A11577)

Immunofluorescence - [KD Validated] EGFR Rabbit pAb (A11577)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KD Validated] EGFR Rabbit pAb (A11577)

Product:

[KD Validated] EGFR Rabbit pAb

Journal:

Viruses

Application:

IF:

5.04

Species:

Homo sapiens

PMID:

32846937

Title:

The Role of Epidermal Growth Factor Receptor Signaling Pathway during Bovine Herpesvirus 1 Productive Infection in Cell Culture

References for [KD Validated] EGFR Rabbit pAb (A11577)

Product:

[KD Validated] EGFR Rabbit pAb

Journal:

Frontiers in oncology

Application:

IF:

5.73

Species:

Homo sapiens

PMID:

36158676

Title:

Co-administration of MDR1 and BCRP or EGFR/PI3K inhibitors overcomes lenvatinib resistance in hepatocellular carcinoma - PMC

References for [KD Validated] EGFR Rabbit pAb (A11577)

Product:

[KD Validated] EGFR Rabbit pAb

Journal:

RNA biology

Application:

IF:

4.76

Species:

Homo sapiens

PMID:

36350790

Title:

A novel strategy for orthogonal genetic regulation on different RNA targeted loci simultaneously - PMC

References for [KD Validated] EGFR Rabbit pAb (A11577)

Product:

[KD Validated] EGFR Rabbit pAb

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Application:

WB, IP

IF:

5.83

Species:

Homo sapiens

PMID:

36468780

Title:

VASN promotes colorectal cancer progression by activating the YAP/TAZ and AKT signaling pathways via YAP

References for [KD Validated] EGFR Rabbit pAb (A11577)

Product:

[KD Validated] EGFR Rabbit pAb

Journal:

eLife

Application:

IF:

8.713

Species:

Homo sapiens, Mus musculus

PMID:

35502895

Title:

METTL3 promotes homologous recombination repair and modulates chemotherapeutic response in breast cancer by regulating the EGF/RAD51 axis

References for [KD Validated] EGFR Rabbit pAb (A11577)

Product:

[KD Validated] EGFR Rabbit pAb

Journal:

Laboratory investigation; a journal of technical methods and pathology

Application:

IF:

5.5

Species:

Mus musculus

PMID:

37039148

Title:

Atractylenolide III Ameliorated Autophagy Dysfunction via Epidermal Growth Factor Receptor-Mammalian Target of Rapamycin Signals and Alleviated Silicosis

References for [KD Validated] EGFR Rabbit pAb (A11577)

Product:

[KD Validated] EGFR Rabbit pAb

Journal:

Microbiology spectrum

Application:

IF:

9.04

Species:

Homo sapiens

PMID:

37655900