Recombinant Human Siglec-8 Protein (RP00333)

Overview

Product name	Recombinant Human Siglec-8 Protein
Catalog No.	RP00333
Description	Recombinant Human Siglec-8 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met17-Ala363) of Human Siglec-8 (Accession #Q9NYZ4) fused with a C-hFc tag at the C-terminus.
Purity	> 95% by SDS-PAGE；> 95% by HPLC
Endotoxin	< 1 EU/μg of the protein by LAL method
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Species	Human
Calculated MW	64.6 kDa

Siglec-8, also known as SAF, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains.Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2, 3-linked sialic acid. Also binds to alpha-2, 6-linked sialic acid.

Product information

Synonym	CDw329; MGC59785; SAF2; SAF2SAF-2; Siglec-8; SIGLEC8L; SIGLEC8
Expression Host	HEK293 cells
Tag	C-hFc
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.