제품 > 항체 > 단일클론항체(mAb)

ABflo® 488 Rabbit anti-Human Siglec-8 mAb (A24358)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry:1X10^6 293F cells (negative control, Left) and 293F (Transfection, right) cells were surface-stained with ABflo® 488 Rabbit anti-Human Siglec-8 mAb(A24358, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgM isotype control (5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 293F (Transfection) cells were surface-stained with ABflo® 488 Rabbit IgM isotype control (5 μl/Test, left) or ABflo® 488 Rabbit anti-Human Siglec-8 mAb(A24358, 5 μl/Test, right).

Overview

Product name	ABflo® 488 Rabbit anti-Human Siglec-8 mAb
Catalog No.	A24358
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgM
CloneNo.	ARC52422-IgM-01-ABf488

Background

Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010]

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 17-363 of human Siglec-8(NP_055257.2)
Sequence	MEGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLNYKTKQLSVFVTALTHRPDILILGTLESGHSRNLTCSVPWACKQGTPPMISWIGASVSSPGPTTARSSVLTLTPKPQDHGTSLTCQVTLPGTGVTTTSTVRLDVSYPPWNLTMTVFQGDATASTALGNGSSLSVLEGQSLRLVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQGSQHISLSLSLQNEGTGTSRPVSQVTLAA
Gene ID
Swiss Prot
Synonyms	SIGLEC8; SAF2; SIGLEC-8; SIGLEC8L; sialic acid-binding Ig-like lectin 8
Calculated MW	44kDa/46kDa/54kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Plasma membrane