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PE Rabbit anti-Human Siglec-8 mAb (A26516)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - PE Rabbit anti-Human Siglec-8 mAb (A26516)

Flow cytometry: 1X10^6 293F cells (negative control, left) and 293F (Transfection, right) cells were surface-stained with PE Rabbit anti-Human Siglec-8 mAb (A26516, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - PE Rabbit anti-Human Siglec-8 mAb (A26516)

Flow cytometry: 1X10^6 293F (Transfection) cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human Siglec-8 mAb (A26516, 5 μl/Test, right).

ABclonal:Flow CytoMetry - PE Rabbit anti-Human Siglec-8 mAb (A26516)

Flow cytometry: 1X10^6 Human peripheral blood granulocytes were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human Siglec-8 mAb (A26516, 5 μl/Test, right).

ABclonal:Flow CytoMetry - PE Rabbit anti-Human Siglec-8 mAb (A26516)

Flow cytometry:1X10^6 Human peripheral blood granulocytes were surface-stained with PE Rabbit anti-Human Siglec-8 mAb (A26516, 5 μl/Test) and PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or APC Mouse anti-Human CCR3 mAb (5 μl/Test, right).

Overview

Product namePE Rabbit anti-Human Siglec-8 mAb
Catalog No.A26516
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC52422-PE
Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 17-363 of human Siglec-8(NP_055257.2).
SequenceMEGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLNYKTKQLSVFVTALTHRPDILILGTLESGHSRNLTCSVPWACKQGTPPMISWIGASVSSPGPTTARSSVLTLTPKPQDHGTSLTCQVTLPGTGVTTTSTVRLDVSYPPWNLTMTVFQGDATASTALGNGSSLSVLEGQSLRLVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQGSQHISLSLSLQNEGTGTSRPVSQVTLAA
Gene ID
Swiss Prot
SynonymsSAF2; SIGLEC-8; SIGLEC8L
Calculated MW44kDa/46kDa/54kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationMembrane, Single-pass type I membrane protein, plasma membrane.

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ABclonal:Flow CytoMetry - PE Rabbit anti-Human Siglec-8 mAb (A26516)}

Flow CytoMetry - PE Rabbit anti-Human Siglec-8 mAb (A26516)

Flow cytometry: 1X10^6 293F cells (negative control, left) and 293F (Transfection, right) cells were surface-stained with PE Rabbit anti-Human Siglec-8 mAb (A26516, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - PE Rabbit anti-Human Siglec-8 mAb (A26516)}

Flow CytoMetry - PE Rabbit anti-Human Siglec-8 mAb (A26516)

Flow cytometry: 1X10^6 293F (Transfection) cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human Siglec-8 mAb (A26516, 5 μl/Test, right).
ABclonal:Flow CytoMetry - PE Rabbit anti-Human Siglec-8 mAb (A26516)}

Flow CytoMetry - PE Rabbit anti-Human Siglec-8 mAb (A26516)

Flow cytometry: 1X10^6 Human peripheral blood granulocytes were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human Siglec-8 mAb (A26516, 5 μl/Test, right).
ABclonal:Flow CytoMetry - PE Rabbit anti-Human Siglec-8 mAb (A26516)}

Flow CytoMetry - PE Rabbit anti-Human Siglec-8 mAb (A26516)

Flow cytometry:1X10^6 Human peripheral blood granulocytes were surface-stained with PE Rabbit anti-Human Siglec-8 mAb (A26516, 5 μl/Test) and PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or APC Mouse anti-Human CCR3 mAb (5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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