제품 > 항체 > 단일클론항체(mAb)

APC Rabbit anti-Human Siglec-8 mAb (A26517)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 293F cells (negative control, left) and 293F (Transfection, right) cells were surface-stained with APC Rabbit anti-Human Siglec-8 mAb (A26517, 5 μl/Test, orange line) or APC Rabbit IgG isotype control (A24173, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 293F (Transfection) cells were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human Siglec-8 mAb (A26517, 5 μl/Test, right).

Flow cytometry: 1X10^6 Human peripheral blood granulocytes were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human Siglec-8 mAb (A26517, 5 μl/Test, right).

Flow cytometry:1X10^6 Human peripheral blood granulocytes were surface-stained with APC Rabbit anti-Human Siglec-8 mAb (A26517, 5 μl/Test) and APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or PE Mouse anti-Human CCR3 mAb (5 μl/Test, right).

Overview

Product name	APC Rabbit anti-Human Siglec-8 mAb
Catalog No.	A26517
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC52422-APC

Background

Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 17-363 of human Siglec-8(NP_055257.2).
Sequence	MEGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLNYKTKQLSVFVTALTHRPDILILGTLESGHSRNLTCSVPWACKQGTPPMISWIGASVSSPGPTTARSSVLTLTPKPQDHGTSLTCQVTLPGTGVTTTSTVRLDVSYPPWNLTMTVFQGDATASTALGNGSSLSVLEGQSLRLVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQGSQHISLSLSLQNEGTGTSRPVSQVTLAA
Gene ID
Swiss Prot
Synonyms	SAF2; SIGLEC-8; SIGLEC8L
Calculated MW	44kDa/46kDa/54kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Membrane, Single-pass type I membrane protein, plasma membrane.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

APC Rabbit anti-Human Siglec-8 mAb images

Flow CytoMetry - APC Rabbit anti-Human Siglec-8 mAb (A26517)

Flow cytometry: 1X10^6 293F (Transfection) cells were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human Siglec-8 mAb (A26517, 5 μl/Test, right).

Flow CytoMetry - APC Rabbit anti-Human Siglec-8 mAb (A26517)

* For research use only. Not for therapeutic or diagnostic purposes.