Overview
Product name | Recombinant Mouse CD24 Protein |
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Catalog No. | RP01437 |
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Description | Recombinant Mouse CD24 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn27-Gly53) of mouse CD24 (Accession #NP_033976.1) fused with a hFc tag at the C-terminus. |
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Bio-Activity | Measured by its binding ability in a functional ELISA.Immobilized Human CD24 (Catalog: RP01437) at 2 μg/mL (100 μL/well) can bind Human Siglec10 with a linear range of 40-900ng/mL.
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Purity | > 95% by SDS-PAGE. |
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Endotoxin | <0.1EU/μg |
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Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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Species | Mouse |
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Calculated MW | 28.69 kDa |
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Background
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 24, also known as signal transducer CD24 or heat stable antigen CD24 (HSA), is a mucin-type glycosylphosphatidylinositol-linked glycoprotein expressed on the surface of B-cells, differentiating neuroblasts and many tumors. It is involved in molecular adhesion and metastatic tumor spread and serve as a normal receptor for P-selectin. The CD24 / P-selectin pathway could be important in disseminating tumor cells by facilitating the interaction with platelet and endothelial cells. It has also been considered as a tumor marker. High rate of CD24 expressions have been found in epithelial ovarian cancer, breast cancer, non-small cell lung cancer, prostate cancer and pancreatic cancer.
Product information
Synonym | CD24; CD24A; FLJ22950; FLJ43543; MGC75043; CD24 |
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Expression Host | HEK293 cells |
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Tag | C-hFc |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.