제품 > 항체 > 단일클론항체(mAb)

CD24 Rabbit mAb (A25473)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of lysates from Mouse liver using CD24 Rabbit mAb (A25473) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:30s.

ABclonal:Immunofluorescence - CD24 Rabbit mAb (A25473)

Confocal imaging of Neuro-2a cells using CD24 Rabbit mAb (A25473, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of paraffin-embedded Mouse speeln tissue using CD24 Rabbit mAb (A25473, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Flow CytoMetry - CD24 Rabbit mAb (A25473)

Flow cytometry: 1X10^6 RAW 264.7 cells (Low Expression, left) and C57BL/6 mouse Splenocytes (right) were surface-stained with CD24 Rabbit mAb (A25473, 2 μg/mL, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line), followed by FITC conjugated goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 C57BL/6 mouse Splenocytes cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or CD24 Rabbit mAb (A25473, 2 μg/mL, right).

Overview

Product name	CD24 Rabbit mAb
Catalog No.	A25473
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC66569

Background

CD24, also know as heat stable antigen HSA, is a P-selectin ligand involved in adhesion. It is a GPI-anchored glycoprotein expressed on many types of cells, including hematopoietic cells, neural cells, and epithelial cells. CD24 is widely used to delineate stages of lymphocyte development . It also binds to Siglec-10 in humans or Siglec-G in mice . CD24 is frequently used as a marker to identify and isolate cancer stem cells in various cancer types .

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 27-53 of mouse CD24 (NP_033976.1).
Sequence	NQTSVAPFPGNQNISASPNPSNATTRG
Gene ID
Swiss Prot
Synonyms	HSA; Cd24; Ly-52; nectadrin
Calculated MW	8kDa
Observed MW	37kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:4000 IF/ICC 1:200 - 1:2000 FC 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Flow Cytometry
Positive samples	Mouse liver
Cellular location	Cell membrane.

Documents

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Research Area

CD24 Rabbit mAb images

Western blot - CD24 Rabbit mAb (A25473)

Immunofluorescence - CD24 Rabbit mAb (A25473)

Flow CytoMetry - CD24 Rabbit mAb (A25473)

Flow cytometry: 1X10^6 C57BL/6 mouse Splenocytes cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or CD24 Rabbit mAb (A25473, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.