제품 > 항체 > 다중클론항체(pAb)

mouse CD24 Rabbit pAb (A24878)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of lysates from Mouse liver using mouse CD24 Rabbit pAb (A24878) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product name	mouse CD24 Rabbit pAb
Catalog No.	A24878
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

CD24 (also known as heat-resistant antigen HSA) is a P-selectin ligand associated with adhesion. It is a GPI anchored glycoprotein expressed in many types of cells, including hematopoietic cells, neural cells, and epithelial cells. CD24 is widely used to determine the stage of lymphocyte development. It also binds to Siglec-10 in human cells or Siglec-G in mouse cells. CD24 is often used as a cancer stem cell marker for detecting and isolating various types of cancer cells.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 27-53 of mouse CD24（NP_033976.1）.
Sequence	NQTSVAPFPGNQNISASPNPSNATTRG
Gene ID
Swiss Prot
Synonyms
Calculated MW	8kDa
Observed MW	Refer to figures

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Flow Cytometry
Positive samples	Mouse liver
Cellular location	Cell membrane.