제품 > 항체 > 단일클론항체(mAb)

ABflo® 488 Rabbit anti-Mouse CD24 mAb (A25470)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Confocal imaging of Neuro-2a cells using ABflo® 488 Rabbit anti-Mouse CD24 mAb (A25470, dilution 1:50). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of paraffin-embedded Mouse spleen tissue using ABflo® 488 Rabbit anti-Mouse CD24 mAb (A25470, dilution 1:50). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CD24 mAb (A25470)

Flow cytometry: 1X10^6 RAW 264.7 cells (Low Expression, left) and C57BL/6 mouse Splenocytes (right) were surface-stained with ABflo® 488 Rabbit anti-Mouse CD24 mAb (A25470, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 C57BL/6 mouse Splenocytes were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse CD24 mAb (A25470, 5 μl/Test, right).

Overview

Product name	ABflo® 488 Rabbit anti-Mouse CD24 mAb
Catalog No.	A25470
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC66569-ABflo488

Background

CD24, also know as heat stable antigen HSA, is a P-selectin ligand involved in adhesion. It is a GPI-anchored glycoprotein expressed on many types of cells, including hematopoietic cells, neural cells, and epithelial cells. CD24 is widely used to delineate stages of lymphocyte development . It also binds to Siglec-10 in humans or Siglec-G in mice . CD24 is frequently used as a marker to identify and isolate cancer stem cells in various cancer types .

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 27-53 of mouse CD24 (NP_033976.1).
Sequence	NQTSVAPFPGNQNISASPNPSNATTRG
Gene ID
Swiss Prot
Synonyms	HSA; Cd24; Ly-52; nectadrin
Calculated MW	8kDa
Observed MW	Refer to figures

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IF/ICC 1:50 - 1:200 FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Immunofluorescence Flow Cytometry
Positive samples
Cellular location	Cell membrane

Documents

Certificate of Compliance

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Research Area

ABflo® 488 Rabbit anti-Mouse CD24 mAb images

Immunofluorescence - ABflo® 488 Rabbit anti-Mouse CD24 mAb (A25470)

Confocal imaging of Neuro-2a cells using ABflo® 488 Rabbit anti-Mouse CD24 mAb (A25470, dilution 1:50). DAPI was used for nuclear staining (Blue). Objective: 100x.

Immunofluorescence - ABflo® 488 Rabbit anti-Mouse CD24 mAb (A25470)

Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CD24 mAb (A25470)

* For research use only. Not for therapeutic or diagnostic purposes.